What question did this study set out to answer?

To review the development of molecular genetic tools, plasmid biology, chromosomal mapping, and gene cloning in Clostridium perfringens to better understand its pathogenic mechanisms.

December 1, 1991Microbiological ReviewsOpen Access

Molecular genetics and pathogenesis of Clostridium perfringens

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Authors

JRJulian I. RoodAustralian Centre for Plant Functional Genomics SCS T ColeJohns Hopkins University

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Implication

Review uncovers genetic mapping and shuttle vector systems in Clostridium perfringens, suggesting new strategies to investigate toxin-mediated bacterial pathogenesis.

Key Points

To review the development of molecular genetic tools, plasmid biology, chromosomal mapping, and gene cloning in Clostridium perfringens to better understand its pathogenic mechanisms.
Characterized bacteriocin plasmids and conjugative R plasmids to construct shuttle vectors capable of replication in both Clostridium perfringens and Escherichia coli.
Constructed a detailed physical map of the Clostridium perfringens chromosome to localize essential genetic determinants.
Developed reproducible plasmid transformation protocols and cloned genes encoding extracellular enzymes and toxins.
Enabled bidirectional genetic transfer between Clostridium perfringens and Escherichia coli using newly constructed shuttle plasmids.
Mapped multiple antibiotic resistance genes and extracellular toxin determinants onto a comprehensive chromosomal physical map.

Cite This Study

Rood et al. (1991) studied this question.

synapsesocial.com/papers/6a715baafebe604dd70a07de https://doi.org/10.1128/mr.55.4.621-648.1991

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Implication

Review uncovers genetic mapping and shuttle vector systems in Clostridium perfringens, suggesting new strategies to investigate toxin-mediated bacterial pathogenesis.

Molecular genetics and pathogenesis of Clostridium perfringens

Discussion

Member takes

Implication

Key Points

Cite This Study

Molecular genetics and pathogenesis of Clostridium perfringens

Discussion

Member takes

Implication

Key Points

Cite This Study

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