May 1, 1992Journal of VirologyOpen Access

Conserved nucleotides in the TAR RNA stem of human immunodeficiency virus type 1 are critical for Tat binding and trans activation: model for TAR RNA tertiary structure

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Population

In vitro and in vivo models of HIV-1 Tat-TAR interaction

Comparison

Nucleotide substitutions and synthetic propyl… vs Wild-type TAR RNA

Design

Preclinical

Authors

UDUlrike DellingCincinnati Children's Hospital Medical Center LRLorne S. ReidCalifornia Institute of Technology RBRichard W. BarnettTherapure Biopharma (Canada)

Discussion

Member takes

Implication

TAR stem substitutions block Tat binding in models; leaves open therapeutic targeting of this interface in human HIV.

Structured PICO

Population

In vitro and in vivo models of HIV-1 Tat-TAR interaction

Intervention

Nucleotide substitutions and synthetic propyl phosphate linkers in the TAR RNA stem

Comparator

Wild-type TAR RNA

Outcome

Complex formation with Tat in vitro and biological activity of TAR in vivosurrogate

Uridine 23 in the TAR RNA bulge forms a stable tertiary interaction with the GC pair directly above it, widening the major groove to expose hydrogen-bonding surfaces for Tat recognition.

Cite This Study

Delling et al. (1992) studied this question.

synapsesocial.com/papers/6a715f6d6c240de38cdc1ca8 https://doi.org/10.1128/jvi.66.5.3018-3025.1992

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Conserved nucleotides in the TAR RNA stem of human immunodeficiency virus type 1 are critical for Tat binding and trans activation: model for TAR RNA tertiary structure

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Conserved nucleotides in the TAR RNA stem of human immunodeficiency virus type 1 are critical for Tat binding and trans activation: model for TAR RNA tertiary structure

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider