Reactivation of Phosphorylated Actin Depolymerizing Factor and Identification of the Regulatory Site

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Population

HeLa cells transfected with chick wild-type ADF cDNA or mutated cDNA

Comparison

Mutation of Ser3 vs Wild-type ADF cDNA or cDNA mutated at Ser24 or…

Design

Preclinical

Authors

Discussion

Clarifies ADF regulation at a single site; leaves open functional consequences for cardiovascular actin dynamics in disease models.

Population

HeLa cells transfected with chick wild-type ADF cDNA or mutated cDNA

Intervention

Mutation of Ser3 (deletion or conversion to Ala or Glu)

Comparator

Wild-type ADF cDNA or cDNA mutated at Ser24 or Thr25

Outcome

Phosphorylation of exogenous ADFsurrogate

Identifies Ser3 as the single in vivo regulatory phosphorylation site for actin depolymerizing factor (ADF).

Agnew et al. (1995) studied this question.

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