Key Points
- To determine the release kinetics of glycogen phosphorylase and creatine kinase into cardiac lymph during circumflex coronary artery occlusion and subsequent reperfusion.
- Monitored cardiac lymph and blood plasma for glycogen phosphorylase and creatine kinase enzymatic activity in conscious dogs during control conditions, circumflex coronary artery occlusion, and reperfusion.
- Evaluated ischemic durations ranging from 10 to over 20 minutes, both with and without reperfusion, accompanied by histological evaluation of myocardial tissue.
- Occlusions as short as 10 minutes prompted an immediate surge of phosphorylase and creatine kinase into cardiac lymph upon reperfusion, whereas enzyme elevations in blood plasma were delayed for several hours.
- Absence of reperfusion led to delayed and later-peaking lymph enzyme release, with total enzyme output scaling alongside the duration of coronary occlusion.
- Histological analysis revealed myocardial infarction in hearts occluded for 20 minutes or longer, while 10-to-15-minute occlusions showed localized cell injury or death in two hearts and no defined infarcts in the remainder.
Structured PICO
IInterventionCircumflex coronary artery (CFX) occlusion (ranging from 10 minutes to 20 minutes or longer) and reperfusion
CComparatorControl state and absence of reperfusion
OOutcomeGlycogen phosphorylase and creatine kinase (CK) enzymatic activity in cardiac lymphsurrogate
In a canine model, cardiac lymph analysis detects myocardial ischemia and reperfusion injury earlier than blood plasma through the immediate appearance of creatine kinase and phosphorylase.