Effects of lymphatic transport of enzyme on plasma creatine kinase time-activity curves after myocardial infarction in dogs.

in vitro and the effects of marked perturbation of cardiac lymph flow in vivo on plasma CK time-activity curves after infarction. Methods ReagentsBovine serum albumin, fraction V, N,N-bis(2-hydroxyethyl) glycine (BICINE), iV-2-hydroxyethylpiperazine-iV-2-ethanesulfonic acid (HEPES), 2-(/V-morpholino)ethanesulfonic acid (MES), and Trasylol were obtained from Calbiochem; iV,iV'-methylenebisacrylamide from Eastman Kodak; indocyanine green dye from Hynson, Westcott and Dunning; ammonium persulfate, bromphenol blue, and sulfosalicylic acid from J.T. Baker Chemicals; ethylenediaminetetraacetic acid, disodium salt (EDTA) from Matheson, Coleman and Bell; Coomassie brilliant blue from Schwarz/Mann; and 2-mercaptoethanol, 5,5'-dithiobis-(2-nitrobenzoic acid) (Ellman's reagent), DL-dithiothreitol (DTT), ethylene-glycol-bis-08-aminoethyl ether) iV.iV'-tetraacetic acid (EGTA), glycine, sodium dodecyl sulfate (SDS), and tris(hydroxymethyl) aminomethane (Tris) from Sigma. Biochemical ProceduresCanine myocardial isoenzyme of MM creatine kinase was isolated and enriched by ethanol fractionation to a specific activity of approximately 400 IU/mg protein.9 Creatine kinase activity was assayed spectrophotometrically as previously described.10 ' " Polyacrylamide disc gel Downloaded from http://ahajournals.org