Why the study?
Does endothelin-1 prevent apoptosis in human vascular endothelial cells via ETA or ETB receptors?
Does endothelin-1 prevent apoptosis in human vascular endothelial cells via ETA or ETB receptors?
Endothelin-1 promotes survival of human vascular endothelial cells primarily through the activation of the ETB receptor and subsequent caspase 3 inhibition.
Answers are grounded in this paper and cite their sources.
ET_B R agonism may aid endothelial survival; leaves open clinical translation in vascular disease.
Our previous work showed the presence of endothelin-1 (ET-1) receptors, ET A and ET B , in human vascular endothelial cells (hVECs). In this study, we wanted to verify whether ET-1 plays a role in the survival of hVECs via the activation of its receptors ET A and (or) ET B (ET A R and ET B R, respectively). Our results showed that treatment of hVECs with ET-1 prevented apoptosis induced by genistein, an effect that was mimicked by treatment with ET B R-specific agonist IRL1620. Furthermore, blockade of ET B R with the selective ET B R antagonist A-192621 prevented the anti-apoptotic effect of ET-1 in hVECs. However, activation of ET A receptor alone did not seem to contribute to the anti-apoptotic effect of ET-1. In addition, the anti-apoptotic effect of ET B R was found to be associated with caspase 3 inhibition and does not depend on the density of this type of receptor. In conclusion, our results showed that ET-1 possesses an anti-apoptotic effect in hVECs and that this effect is mediated, to a great extent, via the activation of ET B R. This study revealed a new role for ET B R in the survival of hVECs.
No takes yet. Share an insight, caveat, or question.
Mikhail et al. (2017) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: