The helicase activity associated with hepatitis C virus nonstructural protein 3 (NS3)
Population
In vitro model using Escherichia coli expressing hepatitis C virus nonstructural protein 3 polypeptide and…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Does not alter HCV management; extends NS3 helicase characterization to guide future antiviral design.
Structured PICO
P
Population
In vitro model using Escherichia coli expressing hepatitis C virus (HCV) nonstructural protein 3 (NS3) polypeptide (amino acids 1175 to 1657) and double-stranded RNA/DNA substrates
I
Intervention
Assessment of RNA and DNA helicase activity
O
Outcome
RNA helicase activity (unwinding of double-stranded RNA substrates)surrogate
HCV NS3 exhibits unique 3' to 5' RNA and DNA helicase activity that is dependent on the polarity of the bound RNA strand.
Cite This Study
Tai et al. (1996) studied this question.