What question did this study set out to answer?

To determine whether mRNA encoding epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) is expressed in human endometrium and decidual tissue.

June 1, 1991Journal of Molecular Endocrinology

Identification of mRNA for epidermal growth factor and transforming growth factor-α present in low copy number in human endometrium and decidua using reverse transcriptase-polymerase chain reaction

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Authors

RHR. E. B. HainingUniversity of Sheffield JSJ. P. SchofieldBradford Royal Infirmary DCD. Stephen Charnock‐JonesUniversity of Cambridge

Discussion

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Implication

Molecular assay reveals EGF and TGF-alpha mRNA in human endometrium and decidua, indicating local production of growth factors during uterine development.

Key Points

To determine whether mRNA encoding epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) is expressed in human endometrium and decidual tissue.
Analyzed tissue fragments from human endometrium, decidua, and peripheral blood fractions (leukocytes and platelets).
Employed reverse transcriptase-polymerase chain reaction (RT-PCR) with nested oligonucleotide primers to amplify low-copy-number mRNA.
Detected mRNA transcripts for both EGF and TGF-alpha in human endometrium and decidua.
Identified no EGF mRNA in peripheral leukocytes or platelets, verifying that EGF expression originates from endometrial tissue.
Observed TGF-alpha mRNA expression in peripheral blood components, precluding definitive confirmation of an exclusively endometrial origin.

Cite This Study

Haining et al. (1991) studied this question.

synapsesocial.com/papers/6a718d4226a7f98052dddb08 https://doi.org/10.1677/jme.0.0060207

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Implication

Molecular assay reveals EGF and TGF-alpha mRNA in human endometrium and decidua, indicating local production of growth factors during uterine development.

Identification of mRNA for epidermal growth factor and transforming growth factor-α present in low copy number in human endometrium and decidua using reverse transcriptase-polymerase chain reaction

Discussion

Member takes

Implication

Key Points

Cite This Study

Identification of mRNA for epidermal growth factor and transforming growth factor-α present in low copy number in human endometrium and decidua using reverse transcriptase-polymerase chain reaction

Discussion

Member takes

Implication

Key Points

Cite This Study

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