Immunohistochemistry for (Pro)renin Receptor in Humans

Figures

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  Figure 1. (Pro)renin receptor staining in organs outside the brain. (A) Thyroid gland; (B) lung; (C) liver; (D) pancreas; and (E) cortex of the adrenal gland. Cells in the zona glomerulosa are stained; (F) medulla of the adrenal gland; (G) kidney; (H) left ventricle; (I) lingual muscle; (J) diaphragm; (K) iliopsoas muscle; (L), aorta; and (M) colon. (a–m) Negative controls in the organs mentioned.

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  Figure 2. (Pro)renin receptor staining in brain lesions. (A) Nucleus tractus solitarii; (B) paraventricular nucleus of the hypothalamus; (C) supraoptic nucleus of the hypothalamus; (D) cerebrum; (E) granular layer of the hippocampus; (F) Purkinje cell layer of the cerebellum; (G) anterior lobe of the pituitary gland; and (H) posterior lobe of the pituitary gland. (a–h) Negative controls in the organs mentioned.

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  Figure 3. Double staining of (pro)renin receptor and growth hormone or adrenocorticotropic hormone. (A), (D) (Pro)renin receptor staining counterstained with hematoxylin; (B) double staining of (pro)renin receptor and growth hormone in the identical section as (A); (C) high-power field of (B); (E) double staining of (pro)renin receptor and adrenocorticotropic hormone in the identical section as (D); and (F) high-power field of (E). Arrows indicate cells costained with (pro)renin receptor.

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  Figure 4. Double staining of (pro)renin receptor and luteinizing hormone or follicle-stimulating hormone. (A), (D) (Pro)renin receptor staining counterstained with hematoxylin; (B) double staining of (pro)renin receptor and luteinizing hormone in the identical section as (A); (C) high-power field of (B); (E) double staining of (pro)renin receptor and follicle-stimulating hormone in the identical section as (D); and (F) high-power field of (E). Arrows indicate cells costained with (pro)renin receptor.

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  Figure 5. Double staining of (pro)renin receptor staining and thyroid-stimulating hormone or prolactin. (A), (D) (Pro)renin receptor staining counterstained with hematoxylin; (B) double staining of (pro)renin receptor and thyroid-stimulating hormone in the identical section as (A); (C) high-power field of (B); (E) double staining of (pro)renin receptor and prolactin in the identical section as (D); and (F) high-power field of (E). Arrows indicate cells costained with (pro)renin receptor.

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  Figure 6. (Pro)renin receptor staining in the granular layer of the hippocampus with (B) or without (A) the human (pro)renin receptor fragment as a blocking peptide. The specificity of the (P)RR antibody used was confirmed.