Key result
Microarray analysis of shear stressed vascular endothelial cells verified earlier findings and identified previously unidentified differentially expressed genes.
cDNA microarray analysis is a useful tool for identifying novel gene expression changes in vascular endothelial cells exposed to mechanical forces like shear stress.
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Supports microarray for endothelial gene discovery under shear stress; leaves open human validation and clinical relevance.
The cDNA microarray is an extremely beneficial tool for study of differential gene expression in the cardiovascular system. This technique is used in many different applications including drug discovery, environmental science, and the effects of mechanical forces on vascular cell phenotype. The paper reviews work by others, and describes our study on effects of shear stress on vascular endothelial cells. These microarray studies verified earlier findings using Northern and polymerase chain reaction (PCR) analyses in this area; and also found previously unidentified differentially expressed genes, leading to new hypotheses regarding how cells and tissues respond to biochemical and mechanical stimuli.
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McCormick et al. (2003) conducted a review in Shear stressed endothelial cells. Shear stress was evaluated on Differential gene expression. Microarray analysis of shear stressed vascular endothelial cells verified earlier findings and identified previously unidentified differentially expressed genes.
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