Key result
Incubation of FMDV Type SAT-2/Egypt/2012 in BHK21 cells yielded maximum infectivity titer at 21 hours and highest complement fixing antigens and 146S protein content at 18 hours at both 35°C and 37°C.
Population
Monolayer BHK21 cell line infected with Foot and mouth disease virus Type SAT-2/Egypt/2012 isolated from…
Design
Preclinical
Follow-up
21 hours
Authors
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May inform SAT-2 FMDV antigen harvest timing; leaves open validation in scaled manufacturing.
The optimal time for harvesting FMDV Type SAT-2/Egypt/2012 in BHK21 cells is 18 hours post-inoculation at 37oC to maximize CF antigens and 146S protein content.
Ali et al. (2013) studied Foot and mouth disease virus (FMDV) Type SAT-2/Egypt/2012. Incubation at 35°C and 37°C was evaluated on Maximum infectivity titer, complement fixing antigens titer, and 146S protein content. Incubation of FMDV Type SAT-2/Egypt/2012 in BHK21 cells yielded maximum infectivity titer at 21 hours and highest complement fixing antigens and 146S protein content at 18 hours at both 35°C and 37°C.
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