Key result
T2-weighted cardiac magnetic resonance imaging successfully quantified the area at risk (32.5 ± 3.1% of left ventricular mass) and infarct size in mice following 20-minute coronary occlusions.
The development of a T2-weighted CMR sequence allows for noninvasive quantification of the area at risk following myocardial infarction in mice, enabling future preclinical studies of novel therapies.
Enables noninvasive AAR assessment in murine MI models; leaves open translation to clinical T2-weighted CMR protocols.
T(2) -weighted, cardiac magnetic resonance imaging (T(2) w CMR) can be used to noninvasively detect and quantify the edematous region that corresponds to the area at risk (AAR) following myocardial infarction (MI). Previously, CMR has been used to examine structure and function in mice, expediting the study of genetic manipulations. To date, CMR has not been applied to imaging of post-MI AAR in mice. We developed a whole-heart, T(2) w CMR sequence to quantify the AAR in mouse models of ischemia and infarction. The ΔB(0) and ΔB(1) environment around the mouse heart at 7 T were measured, and a T(2) -preparation sequence suitable for these conditions was developed. Both in vivo T(2) w and late gadolinium enhanced CMR were performed in mice after 20-min coronary occlusions, resulting in measurements of AAR size of 32.5 ± 3.1 (mean ± SEM)% left ventricular mass, and MI size of 50.1 ± 6.4% AAR size. Excellent interobserver agreement and agreement with histology were also found. This T(2) w imaging method for mice may allow for future investigations of genetic manipulations and novel therapies affecting the AAR and salvaged myocardium following reperfused MI.
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Beyers et al. (2011) studied Myocardial infarction. T2-weighted cardiac magnetic resonance imaging was evaluated on Area at risk (AAR) size and MI size. T2-weighted cardiac magnetic resonance imaging successfully quantified the area at risk (32.5 ± 3.1% of left ventricular mass) and infarct size in mice following 20-minute coronary occlusions.
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