What question did this study set out to answer?

To evaluate the effect of ouabain on calcium release from isolated cardiac sarcoplasmic reticulum vesicles during changes in ionic environment and caffeine exposure.

January 1, 1979The Japanese Journal of PharmacologyOpen Access

Ouabain potentiation of Ca release from fragmented cardiac sarcoplasmic reticulum.

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Population

Fragmented sarcoplasmic reticulum (FSR) isolated from cat, guinea pig, and frog cardiac muscles

Design

Preclinical

Authors

SFSumiko FujinoSapporo Medical University TITakashi IgarashiKanazawa University KHKatsuji HoshiHokkaido Pharmaceutical University

Discussion

Member takes

Implication

May explain ouabain inotropy in animals; leaves open human translation and clinical relevance.

Key Points

To evaluate the effect of ouabain on calcium release from isolated cardiac sarcoplasmic reticulum vesicles during changes in ionic environment and caffeine exposure.
Isolated fragmented sarcoplasmic reticulum (FSR) microsomal fractions from cat, guinea pig, and frog cardiac muscle using differential ultracentrifugation at 8,000 × g to 35,000 × g.
Loaded FSR with calcium in a potassium methan-sulfonate medium, trapped vesicles on Millipore filters, and washed with potassium chloride medium or 30 mM caffeine to induce calcium release.
Exposed filter-bound vesicles to ouabain (2 × 10⁻⁶ M) for a duration of one minute during ionic environment modification.
Observed nearly identical baseline levels of calcium release from fragmented sarcoplasmic reticulum among cat, guinea pig, and frog cardiac tissue preparations.
Ouabain treatment markedly potentiated calcium release triggered by altering the ionic wash environment in all tested species.

Structured PICO

Population

Fragmented sarcoplasmic reticulum (FSR) isolated from cat, guinea pig, and frog cardiac muscles

Intervention

Ouabain (2 × 10-6 M) treatment for 1 min

Outcome

Ca release from FSRsurrogate

Ouabain potentiates calcium release from the sarcoplasmic reticulum, suggesting this mechanism contributes to its positive inotropic action in cardiac muscle.

Cite This Study

Fujino et al. (1979) studied this question.

synapsesocial.com/papers/6a71b74fac440176ef2a4ebd https://doi.org/10.1254/jjp.29.839

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Ouabain potentiation of Ca release from fragmented cardiac sarcoplasmic reticulum.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Ouabain potentiation of Ca release from fragmented cardiac sarcoplasmic reticulum.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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