July 15, 1997Circulation

Adenovirus-Mediated Gene Transfer Reconstitutes Depressed Sarcoplasmic Reticulum Ca ²⁺ -ATPase Levels and Shortens Prolonged Cardiac Myocyte Ca ²⁺ Transients

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Why the study?

Does SERCA2 adenovirus infection improve Ca2+ transients and SR Ca2+ uptake in PMA-treated neonatal rat cardiac myocytes?

Structured PICO

Does SERCA2 adenovirus infection improve Ca2+ transients and SR Ca2+ uptake in PMA-treated neonatal rat cardiac myocytes?

Population

Cultured neonatal rat cardiac myocytes (NM) treated with phorbol-12-myristate-13-acetate (PMA) to decrease endogenous SERCA2 expression

Intervention

SERCA2 adenovirus infection

Comparator

PMA-treated cells without SERCA2 adenovirus infection (control)

Outcome

SERCA2 mRNA and protein levels, time for decline of Indo-1 Ca2+ transient to half-maximum levels (t(1/2) [Ca2+]i), and oxalate-facilitated SR Ca2+ uptakesurrogate

Adenovirus-mediated SERCA2 gene transfer can reconstitute depressed SERCA2 levels and normalize calcium handling in a cellular model of heart failure.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Supports SERCA2 gene transfer to normalize calcium handling in HF models; leaves open in vivo efficacy and clinical translation.

Cite This Study

Giordano et al. (1997) studied this question.

synapsesocial.com/papers/6a71c7f931a3df824329cfb1 https://doi.org/10.1161/01.cir.96.2.400

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Adenovirus-Mediated Gene Transfer Reconstitutes Depressed Sarcoplasmic Reticulum Ca ²⁺ -ATPase Levels and Shortens Prolonged Cardiac Myocyte Ca ²⁺ Transients

Structured PICO

Discussion

Implication

Cite This Study

Adenovirus-Mediated Gene Transfer Reconstitutes Depressed Sarcoplasmic Reticulum Ca ²⁺ -ATPase Levels and Shortens Prolonged Cardiac Myocyte Ca ²⁺ Transients

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider