Development of a new RT-PCR with multiple primers for detecting Southern African Territories foot-and-mouth disease viruses

Figures

• 

  Fig. 1. The nucleotide identity of 1D genes of all 31 selected representative strains of FMDV SATs and C with complete genomic sequences deposited in GenBank

• 

  Fig. 2. FMD SAT-D8 PCR results with 27 synthesised 1D2A2B genes (cloned into pUC57 vector) of the selected representative strains of FMDV SATs as the templates. Lanes: M – DL2000 marker (2000, 1000, 750, 500, 250, 100bp), 1 – SAT1-AY593850, 2 – SAT1-AY593852, 3 – SAT1-AY593853, 4 – SAT2-KU821592, 5 – SAT2-KM268900, 6 – SAT2-KR108955, 7 – SAT3-KR108950, 8 – SAT3-KJ820999, 9 – SAT3-KM268901, 10 – SAT1-AY593839, 11 – SAT1-AY593840, 12 – SAT1-AY593841, 13 – SAT1-AY593846, 14 – SAT1-AY593838, 15 – SAT1-HM067706, 16 – SAT1-JF749860, 17 – SAT1-AY593842, 18 – SAT2-JF749864, 19 – SAT2-AF540910, 20 – SAT2-AY593848, 21 – SAT2-JF749861, 22 – SAT2-JF749862, 23 – SAT2-AY593847, 24 – SAT2-HM067705, 25 – SAT2-AJ251473, 26 – SAT2-JHM067704, 27 – SAT2-KC440884

• 

  Fig. 3. Analytical sensitivity of FMD SAT-D8 PCR assay with the synthesised 1D2A2B genes of FMDV SATs (cloned into pUC57 vector) as the templates. A – SAT1 strain (GenBank accession number: AY593846), B – SAT2 strain (JF749862), C – SAT3 strain (AY593852). Lanes 1–9 – PCR products of 1D2A2B genes of SATs with concentrations of 4 × 108– 4 × 100 copies/reaction, lane 10 – negative control

• 

  Fig. 4. Electrophoresis of in vitro transcription products of the synthesised 1D2A2B genes (cloned into pUC57 vector) of the representative strains of FMDV SATs registered in GenBank. M – DL2000 Marker, 1 – SAT1-AY593846, 2 – SAT2-JF749862, 3 – SAT3-AY593852

• 

  Fig. 5. Analytical sensitivity of FMD SAT-D8 one-step RT-PCR assay with RNA transcripts of 1D2A2B genes of the representative strains of FMDV SATs as the templates. Lanes: 1–10 – RT-PCR products of RNA transcripts of 1D2A2B genes of FMDV SATs with concentrations of 4 × 109– 4 × 100 copies/reaction. A – SAT1 strain (GenBank accession no. AY593846); B – SAT2 strain (JF749862); C – SAT3 strain (AY593852)

• 

  Fig. 6. FMDV SAT-D8 one-step RT-PCR products of RNA samples extracted from FMDV SATs inactivated antigens and RNA transcripts of the correspondingly synthesised 1D2A2B genes of the reference strains of FMDV SATs registered in GenBank as positive controls. Lanes: M – DL2000 marker, 1 – SAT1-AY593839, 2 – SAT1-AY593841, 3, 4 – SAT1 inactivated antigen, 5, 6 – Puc57 plasmid as negative control, 7 – SAT2 inactivated antigen, 8 – SAT3 inactivated antigen, 9 – SAT2-AY593861, 10 – SAT3-AY593850