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January 1, 1985ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam)Open Access

The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA 3′ termini

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Population

Mammalian mRNA 3' termini and bacterial chloramphenicol acetyl transferase gene constructs linked to an HSV…

Comparison

Deletions in the 'terminator' fragment… vs Undeleted plasmids (wild-type terminator)

Design

Preclinical

Authors

JMJohn McLauchlanMRC University of Glasgow Centre for Virus ResearchDGDairena GaffneyTrinity College DublinJWJ. Lindsay WhittonScripps Research Institute

Discussion

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Implication

YGTGTTYY consensus is essential for efficient mRNA 3' termini formation; extends polyadenylation mechanisms but leaves open cardiac transcript applications.

Structured PICO

P
Population
Mammalian mRNA 3' termini and bacterial chloramphenicol acetyl transferase (CAT) gene constructs linked to an HSV 'terminator' fragment
I
Intervention
Deletions in the 'terminator' fragment, specifically removing the YGTGTTYY consensus sequence
C
Comparator
Undeleted plasmids (wild-type terminator)
O
Outcome
CAT activities and levels of CAT mRNA 3' terminisurrogate

The consensus sequence YGTGTTYY located downstream from the AATAAA signal is essential for the efficient formation of mRNA 3' termini.

Cite This Study

McLauchlan et al. (1985) studied this question.

synapsesocial.com/papers/6a71ca888031ec7bb1dd1cc2https://doi.org/10.1093/nar/13.4.1347
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Also Consider

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  1. 1DNA sequence elements required for regulated expression of the HSV-1 glycoprotein D gene lie within 83 bp of the RNA capsites1983 · 81 citations
  2. 2Mapping of RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15 S β-globin mRNA precursor and mature 10 S β-globin mRNA have identical map coordinates1979 · 1,286 citations
  3. 3Nucleotide sequences from the adenovirus-2 genome.1982 · 327 citations
  4. 4Multiple polyadenylation sites in a mouse α-amylase gene1981 · 183 citations
  5. 5The nucleotide sequence of an untranslated but conserved domain at the 3′ end of the avian sarcoma virus genome1980 · 57 citations