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March 1, 2022journal of ilam university of medical sciencesOpen Access

Investigation of the Effect of Polycystic Ovary Syndrome on the Expression of the Sirtuin-3 Gene in the Ovum of the NMRI Mice

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Key result

Induction of polycystic ovary syndrome significantly reduced the expression of the Sirtuin-3 gene in the ova of NMRI mice compared to healthy controls (ratio 0.18; P<0.05).

Why the study?

Elevated free radicals in ova reduce fertilization quality and are increased in polycystic ovary syndrome, but the expression of the mitochondrial antioxidant gene Sirtuin-3 in affected ova required investigation.

Does polycystic ovary syndrome reduce the expression of the Sirtuin-3 gene in the ova of NMRI mice?

Comparison

Estradiol valerate-induced polycystic ovary syndrome vs control

Design

Animal experimental study

Authors

HJHanieh JalaliMAMahnaz AzarniaFKFatemeh Kamalipour

Discussion

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Member takes

Overview

Sirtuin-3 expression in PCOS mouse ova remains hypothesis-generating; human studies needed before any clinical relevance.

Structured PICO

Does polycystic ovary syndrome reduce the expression of the Sirtuin-3 gene in the ova of NMRI mice?

P
Population
12 female NMRI mice, aged six weeks, were used to investigate the effect of induced polycystic ovary syndrome on Sirtuin-3 gene expression.
I
Intervention
Polycystic ovary syndrome induction via injection of estradiol valerate (40 mg/kg)
C
Comparator
Healthy control mice
O
Outcome
Expression of the Sirtuin-3 gene in the ovasurrogate

Main Result

Effect estimate: ratio of 0.18

p-value: p=<0.05

Polycystic ovary syndrome reduces the expression of the Sirtuin-3 gene in the ova of mice, which may contribute to oxidative stress and reduced fertilization quality.

Cite This Study

Jalali et al. (2022) studied Polycystic ovary syndrome (n=12). Estradiol valerate (PCOS induction) vs. Healthy control mice was evaluated on Expression of Sirtuin-3 gene in the ova (ratio of 0.18, p=<0.05). Induction of polycystic ovary syndrome significantly reduced the expression of the Sirtuin-3 gene in the ova of NMRI mice compared to healthy controls (ratio 0.18; P<0.05).

synapsesocial.com/papers/6a71e42bb5c1fe5ca9dfe9e0https://doi.org/10.52547/sjimu.30.1.1
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1SIRT3 protects endometrial receptivity in patients with polycystic ovary syndrome2024 · 2 citations
  2. 2Mechanistic Study of MiR-30c-5p Regulation of SIRT Expression in Polycystic Ovary Syndrome2025
  3. 3Effect of irisin on ovarian phosphatidylinositol‐3‐kinase/protein kinase B signaling pathway and mitogen‐activated protein kinase/extracellular signal‐regulated kinase pathways of rats with polycystic ovary syndrome2024 · 2 citations
  4. 4Mechanistic Investigation of Vitexin in Ameliorating Ovarian Fibrosis in PCOS Mice via the NR4A1/NLRP3 Signaling Pathway2026
  5. 5HISTOMORPHOLOGICAL AND MOLECULAR CHANGES IN OVARIAN TISSUE IN AN EXPERIMENTAL MODEL OF POLYCYSTIC OVARY SYNDROME-LIKE DISORDERS.2026