Key result
Induction of polycystic ovary syndrome reduced the expression of the Sirtuin-3 gene in the ova of NMRI mice compared to healthy controls.
Why the study?
Elevated free radicals in ova reduce fertilization quality and are increased in polycystic ovary syndrome, but the expression of the mitochondrial antioxidant gene Sirtuin-3 in affected ova required investigation.
Does polycystic ovary syndrome reduce the expression of the Sirtuin-3 gene in the ova of NMRI mice?
Does polycystic ovary syndrome reduce the expression of the Sirtuin-3 gene in the ova of NMRI mice?
Polycystic ovary syndrome reduces the expression of the Sirtuin-3 gene in the ova of mice, which may contribute to oxidative stress and reduced fertilization quality.
Answers are grounded in this paper and cite their sources.
Sirtuin-3 expression in PCOS mouse ova remains hypothesis-generating; human studies needed before any clinical relevance.
Introduction: Increasing levels of free radicals in the ova reduce the quality of their fertilization. Investigations have revealed the elevated production of tissue and serum-free radicals in women with polycystic ovary syndrome. Sirtuin-3 is located in mitochondria and plays an important role in the deactivation of free radicals. This study aimed to investigate the expression of the Sirtuin-3 gene in the ova of polycystic ovary syndrome affected mice. Material & Methods: Polycystic ovary syndrome was induced by the injection of estradiol valerate (40 mg/kg) in six NMRI mice (age: six weeks old; weight: 255); moreover, six mice were selected as a control group. Following the induction, the structure of the ovaries was examined using the histotechnique, and the serum levels of sex hormones were determined using ELISA. The ova were collected from the ovaries, and their RNA was extracted by a special kit and converted to cDNA. Real-Time PCR was performed to analyze the expression of Sirtuin-3, and the Gapdh gene was selected as an internal control. (Ethic code: 97/6505) Findings: In the mice with polycystic ovary syndrome, hydatid cysts were formed in the ovaries. Furthermore, serum FSH hormone levels decreased in the affected mice, while LH and testosterone levels elevated, compared to those in the control mice (P<0.001). The expression of Sirtuin-3 was reduced in the ova of mice with polycystic ovary syndrome, compared to that in healthy mice in the ratio of 0.18 (P<0.05).
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Jalali et al. (2022) studied Polycystic ovary syndrome (n=12). Estradiol valerate (PCOS induction) vs. Healthy control mice was evaluated on Expression of Sirtuin-3 gene in the ova (ratio of 0.18, p=<0.05). Induction of polycystic ovary syndrome reduced the expression of the Sirtuin-3 gene in the ova of NMRI mice compared to healthy controls.
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