Key result
Introduction of an Ala cluster in place of the native interface in period 2 and/or period 3 sites of tropomyosin increased the affinity or cooperativity of actin binding.
Population
Recombinant rat striated muscle alpha-tropomyosins expressed in E. coli and phalloidin-stabilized F-actin
Comparison
Mutagenesis of tropomyosin to introduce… vs Wildtype recombinant striated muscle…
Design
Preclinical
Authors
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Hypothesis-generating for sarcomeric protein design; leaves open effects on cardiac function in vivo.
Destabilization of the coiled-coil interface in specific regions of tropomyosin increases its affinity and cooperativity for binding actin, suggesting local disorder is important for protein-protein interactions.
Singh et al. (2009) studied this question. Tropomyosin mutagenesis (P2Shift, P3Shift, P5RP3) vs. Wildtype tropomyosin was evaluated on Actin affinity and cooperativity of binding. Introduction of an Ala cluster in place of the native interface in period 2 and/or period 3 sites of tropomyosin increased the affinity or cooperativity of actin binding.
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