In vitro infection of murine bone marrow cultures with F-MuLV reproduces the course of in vivo leukemogenesis, providing a model for studying preleukemic stages.
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No immediate clinical implications; extends in vitro tools for preleukemic research but leaves translation open.
A Friend helper virus I-5(F-MuLV) which induces myeloblastic leukemias in mice after a latency of several months, was used to infect long-term bone marrow cultures. From 48 to 71 weeks after in vitro infection, 4/14 cultures gave rise to transplantable malignant myeloblastic cells. These cells were shown to genuinely result from as in vitro transformation of virus-infected normal bone marrow cells. The in vitro transformation reproduced the course of the in vivo disease. It provided unique material for in vitro investigation of the preleukemic stages of long-term leukemogenesis. Successive cellular events were: (1) freezing of the normal myelomonocytic differentiation process; (2) change from factor-dependent to an autonomous growth; (3) acquisition of in vivo tumorigenicity.
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Heard et al. (1983) studied this question.
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