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April 1, 2005Applied and Environmental MicrobiologyOpen Access

Rapid and Sensitive Detection of Noroviruses by Using TaqMan-Based One-Step Reverse Transcription-PCR Assays and Application to Naturally Contaminated Shellfish Samples

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Why the study?

Does a one-step TaqMan RT-PCR assay improve the detection sensitivity of Noroviruses in stool and shellfish samples compared to existing real-time and nested PCR assays?

Population

84 stool specimens and 38 shellfish cDNA extracts

Comparison

One-step TaqMan reverse transcription-PCR assays… vs A recently described real-time assay and nested…

Design

Other

Authors

NJN. JothikumarCenters for Disease Control and PreventionJLJames LowtherCentre for Environment, Fisheries and Aquaculture ScienceKHKathleen HenshilwoodMarine Institute

Discussion

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Implication

Supports rapid Norovirus detection in clinical and environmental samples; leaves open prospective validation before routine adoption.

Structured PICO

Does a one-step TaqMan RT-PCR assay improve the detection sensitivity of Noroviruses in stool and shellfish samples compared to existing real-time and nested PCR assays?

P
Population
84 stool specimens (including NoV strains representing 19 different genotypes: 7 GI, 11 GII, and 1 GIV strains) and 38 shellfish cDNA extracts
I
Intervention
One-step TaqMan reverse transcription (RT)-PCR assays for the detection of genogroup I (GI) and GII Noroviruses
C
Comparator
A recently described real-time assay and nested PCR
O
Outcome
Sensitivity and specificity for Norovirus detectionsurrogate

The developed one-step TaqMan RT-PCR assay provides a rapid, sensitive, and reliable method for routine monitoring of Noroviruses in clinical and environmental samples.

Cite This Study

Jothikumar et al. (2005) studied this question.

synapsesocial.com/papers/6a71f3316ceb2bbd16e04357https://doi.org/10.1128/aem.71.4.1870-1875.2005
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