What question did this study set out to answer?

This study aims to examine how carvedilol affects PDGF-induced signaling in human cardiac fibroblasts.

April 1, 2002Journal of Cardiovascular Pharmacology

Carvedilol Inhibits Platelet-Derived Growth Factor-Induced Signal Transduction in Human Cardiac Fibroblasts

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Why the study?

Does carvedilol inhibit PDGF-induced signal transduction and mitogenesis in human cardiac fibroblasts?

Population

Human cardiac fibroblasts cultured from myocardial biopsy samples taken from patients with idiopathic…

Comparison

Carvedilol (1, 5, 10, and 20 microM) vs Other beta-adrenoceptor antagonists and PDGF…

Design

Preclinical

Authors

ULUlrich LotzeUniversity of Cape Town SHStefan HeinkeFriedrich Schiller University Jena MFMichael FritzenwangerGoethe University Frankfurt

Discussion

Member takes

Implication

Carvedilol may inhibit fibroblast mitogenesis via PDGF; leaves open any clinical anti-fibrotic role in heart failure.

Key Points

This study aims to examine how carvedilol affects PDGF-induced signaling in human cardiac fibroblasts.
Human cardiac fibroblasts cultured from myocardial biopsies of idiopathic dilated cardiomyopathy patients.
Investigation of PDGF-induced PDGF-beta receptor kinase stimulation and carvedilol's inhibitory effects using Western blotting and colorimetric assays.
Evaluation of carvedilol concentrations (1, 5, 10, 20 microM) on DNA synthesis and PDGF receptor tyrosine phosphorylation.
Carvedilol (5 microM) inhibited PDGF receptor kinase stimulation and reduced DNA synthesis by approximately 50%.
Higher concentrations (10 and 20 microM) inhibited PDGF-stimulated mitogenesis by 64% and 75%, respectively.
Propranolol and metoprolol did not significantly affect PDGF-induced receptor autophosphorylation.

Structured PICO

Does carvedilol inhibit PDGF-induced signal transduction and mitogenesis in human cardiac fibroblasts?

Population

Human cardiac fibroblasts cultured from myocardial biopsy samples taken from patients with idiopathic dilated cardiomyopathy

Intervention

Carvedilol (1, 5, 10, and 20 microM)

Comparator

Other beta-adrenoceptor antagonists (propranolol 10 microM and metoprolol 10 microM) and PDGF stimulation alone

Outcome

PDGF-induced PDGF receptor tyrosine phosphorylation and DNA synthesissurrogate

Carvedilol inhibits PDGF-stimulated mitogenesis and receptor autophosphorylation in human cardiac fibroblasts, providing a potential mechanism for its anti-fibrotic effects in chronic heart failure.

Cite This Study

Lotze et al. (2002) studied this question.

synapsesocial.com/papers/6a720747a2d7cf2e39c2e358 https://doi.org/10.1097/00005344-200204000-00014

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Carvedilol Inhibits Platelet-Derived Growth Factor-Induced Signal Transduction in Human Cardiac Fibroblasts

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Carvedilol Inhibits Platelet-Derived Growth Factor-Induced Signal Transduction in Human Cardiac Fibroblasts

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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