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December 1, 1995Journal of VirologyOpen Access

Analysis of picornavirus 2A(pro) proteins: separation of proteinase from translation and replication functions

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Population

Poliovirus genome manipulated by replacing its 2A-encoding sequence with the corresponding sequence of…

Comparison

Chimeric PV genomes and introduction of specific… vs Wild-type PV genome (implied)

Design

Preclinical

Authors

HLH H LuChinese Preventive Medicine AssociationXLX LiState University of New YorkACAndrea CuconatiRutgers, The State University of New Jersey

Discussion

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Implication

CBV4 2A(pro) functionally substitutes for PV in chimeras with targeted mutations; extends enterovirus compatibility data but leaves open any translational applications.

Structured PICO

P
Population
Poliovirus (PV) genome manipulated by replacing its 2A-encoding sequence with the corresponding sequence of coxsackie B4 virus (CBV4) or human rhinovirus type 2 (HRV2), tested in vitro and in transfected HeLa cells
I
Intervention
Chimeric PV genomes (PV/CBV4-2A and PV/HRV2-2A) and introduction of specific single-amino-acid mutations
C
Comparator
Wild-type PV genome (implied)
O
Outcome
Viral viability and growth pattern (plaque phenotype)surrogate

The 2A(pro) protein of the closely related enterovirus CBV4 can functionally substitute for that of PV in vivo, and specific genetic modifications can drastically improve the growth of the chimeric virus.

Cite This Study

Lu et al. (1995) studied this question.

synapsesocial.com/papers/6a7212e52163a0a01bc5ec7dhttps://doi.org/10.1128/jvi.69.12.7445-7452.1995
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Defective RNA replication by poliovirus mutants deficient in 2A protease cleavage activity1995 · 45 citations
  2. 2Purification of two picornaviral 2A proteinases: Interaction with eIF-4.gamma. and influence on the in vitro translation1993 · 181 citations
  3. 3Construction and genetic analysis of dicistronic polioviruses containing open reading frames for epitopes of human immunodeficiency virus type 1 gp1201995 · 38 citations
  4. 4Relationship of p220 cleavage during picornavirus infection to 2A proteinase sequencing1988 · 144 citations
  5. 5Poliovirus Protein 3CD Is the Active Protease for Processing of the Precursor Protein P1 in vitro1988 · 188 citations