Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
July 1, 1993Biochemistry

Purification of two picornaviral 2A proteinases: Interaction with eIF-4.gamma. and influence on the in vitro translation

View Full Paper
Ask AI
Bookmark
Share

Population

Escherichia coli expressing mature proteinases 2A of human rhinovirus 2 and coxsackievirus B4, and HeLa cell…

Comparison

Addition of purified HRV2 2A proteinase vs Addition of purified eIF-4 or baseline…

Design

Preclinical

Authors

HLHans LiebigUniversity of Veterinary Medicine ViennaEZErika ZieglerSaarland UniversityRYRiqiang YanUConn Health

Discussion

Loading...

Member takes

Implication

May inform antiviral strategies targeting translation shutoff; leaves open in vivo relevance and therapeutic translation.

Structured PICO

P
Population
Escherichia coli expressing mature proteinases 2A of human rhinovirus 2 and coxsackievirus B4, and HeLa cell cytoplasmic translation extracts
I
Intervention
Addition of purified HRV2 2A proteinase
C
Comparator
Addition of purified eIF-4 or baseline translation extracts
O
Outcome
eIF-4 gamma cleavage and translation efficiency of capped mRNA versus uncapped viral RNAsurrogate

Purified picornaviral 2A proteinases directly cleave eIF-4 gamma, inhibiting host capped mRNA translation while enhancing viral RNA translation.

Cite This Study

Liebig et al. (1993) studied this question.

synapsesocial.com/papers/6a8212646cfba4e31a9fd869https://doi.org/10.1021/bi00080a033
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The p220 component of eukaryotic initiation factor 4F is a substrate for multiple calcium-dependent enzymes1990 · 18 citations
  2. 2Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei1983 · 11,637 citations
  3. 3Relationship of eukaryotic initiation factor 3 to poliovirus-induced p220 cleavage activity1992 · 57 citations
  4. 4Inhibition of translation in cells infected with a poliovirus 2Apro mutant correlates with phosphorylation of the alpha subunit of eucaryotic initiation factor 21989 · 82 citations
  5. 5Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eucaryotic initiation factor 3 and a cap binding protein complex.1982 · 590 citations