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February 1, 1987Journal of Biological ChemistryOpen Access

Transport of beta-very low density lipoproteins and chylomicron remnants by macrophages is mediated by the low density lipoprotein receptor pathway.

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To investigate whether the receptor-mediated uptake of beta-very low density lipoproteins and chylomicron remnants by macrophages is mediated by the low density lipoprotein receptor pathway.
Examined uptake of radiolabeled 125I-beta VLDL and 125I-chylomicron remnants in monolayer cultures of J774 and P388D1 macrophage cell lines and primary mouse peritoneal macrophages.
Conducted competition assays using unlabeled human LDL, human acetyl-LDL, and polyclonal anti-LDL receptor IgG raised against rat liver LDL receptor.
Characterized receptor binding using nitrocellulose ligand and immunoblotting on detergent-solubilized membranes from mouse macrophages, fibroblasts, and liver compared to rat liver.
Excess unlabeled human LDL reduced uptake of 125I-beta VLDL and 125I-chylomicron remnants by 80% to 90%, whereas acetyl-LDL produced no significant competition.
Specific anti-LDL receptor IgG competitively inhibited uptake by greater than 80% and bound to two membrane proteins of 125,000 and 111,000 Da, with 70% to 90% of lipoprotein binding confined to the 125,000-Da peptide.
Sterol suppression via 48-hour incubation with 25-hydroxycholesterol and cholesterol reduced 125I-beta VLDL uptake and receptor blotting by only 25%.

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Implication

In vitro study reveals uptake of beta-VLDL and chylomicron remnants via the LDL receptor pathway in mouse macrophages, indicating a conserved lipoprotein clearance mechanism.

Cite This Study

Ellsworth et al. (1987) studied this question.

synapsesocial.com/papers/6a721c6a35aa2c282ce31779 https://doi.org/10.1016/s0021-9258(18)61656-4

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Implication

In vitro study reveals uptake of beta-VLDL and chylomicron remnants via the LDL receptor pathway in mouse macrophages, indicating a conserved lipoprotein clearance mechanism.

Transport of beta-very low density lipoproteins and chylomicron remnants by macrophages is mediated by the low density lipoprotein receptor pathway.

Key Points

Discussion

Implication

Cite This Study

Transport of beta-very low density lipoproteins and chylomicron remnants by macrophages is mediated by the low density lipoprotein receptor pathway.

Key Points

Discussion

Implication

Cite This Study

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