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Molecular markers were employed to the characterization and analysis of hemp genetic structure by using RAPD technique. The results are presented about the statistical treatment of the molecular data. In addition, markers tightly linked to the male sex and their applications are discussed, and a short protocol for direct amplification of such markers from hemp tissue is provided. A molecular map of hemp, including a number of RAPD markers obtained from a progeny of a cross between a female Carmagnola plant and a monoecious accession is also presented. Finally, the state of the art of sex genetics in hemp and the possibilities of developing molecular markers linked to different hemp chemotypes are discussed.
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Mandolino et al. (2002) studied this question.
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