Characterisation of humoral trophic factors involved in intestinal adaptation using a 3T3 cell growth factor assay.

Q: What is the clinical evidence from this study?

Population: Intestinal adaptation following intestinal resection. Intervention: 80% intestinal resection or peritoneal incision vs. No surgery or dorsal slit. Primary outcome: Stimulation of DNA synthesis in 3T3 fibroblast cells and ileal DNA specific activity.

Q: What does this research mean for the field?

Platelet-free plasma from rats undergoing intestinal resection or peritoneal incision stimulates cell proliferation via circulating trophic factors of 6,000 to 14,000 Da. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

Jeffrey A. Biller; Robert K. Montgomery; Richard J. Grand; Michael Klagsbrun; Andrew S. Rosenthal

doi:10.1136/gut.28.suppl.243

January 1, 1987GutOpen Access

Characterisation of humoral trophic factors involved in intestinal adaptation using a 3T3 cell growth factor assay.

View Full Paper

Ask AI

Bookmark

Share

Key result

Post-resection rat plasma stimulates cell DNA synthesis via circulating low-molecular-weight trophic factors.

Why the study?

Indirect evidence suggested that circulating trophic factors are involved in intestinal adaptation, prompting the use of a 3T3 fibroblast cell culture system to more directly delineate their presence.

Does diagnosis and treatment of coronary microvascular endothelial dysfunction improve quality of life in patients with chest pain and non-obstructive CAD?

Key Points

To identify and characterize circulating humoral trophic factors that regulate gut mucosal growth and adaptation after small bowel resection.
Assigned rats to four surgical groups: unoperated controls (C-unop), dorsal slit sham controls (S), peritoneal incision controls (C-op), and 80% intestinal resection (R).
Harvested platelet-free plasma at 24 hours, 1 week, and 2 weeks postoperatively to stimulate 3T3 fibroblast DNA synthesis and evaluate ileal DNA specific activity.
Characterized active growth factor fractions by estimating molecular weight, testing heat sensitivity at 60°C, and assessing reduction resistance with dithiothreitol (DTT).
Platelet-free plasma from both the peritoneal incision (C-op) and 80% resection (R) groups stimulated 3T3 fibroblast DNA synthesis across all time points (24 hours, 1 week, and 2 weeks), coinciding with increased ileal DNA specific activity.
Growth factor activity localized to a plasma fraction with a molecular weight between 6,000 and 14,000 Da.
The circulating factor retained activity after reduction with DTT and was partially inactivated following exposure to 60°C heat.

Structured PICO

Does diagnosis and treatment of coronary microvascular endothelial dysfunction improve quality of life in patients with chest pain and non-obstructive CAD?

P

Population

Rats divided into four surgical groups (no surgery, dorsal slit, peritoneal incision, or 80% intestinal resection) evaluated at 24 hours, 1 week, and 2 weeks postoperatively.

I

Intervention

Diagnosis and treatment of coronary microvascular endothelial dysfunction

C

Comparator

Patients with normal endothelial function

O

Outcome

Quality of life assessed by the SF-36 surveypatient reported

Ask about this paper

Answers are grounded in this paper and cite their sources.

Implication

Diagnosis and treatment of coronary microvascular endothelial dysfunction in patients with angina and non-obstructive CAD is associated with better long-term quality of life.

Abstract

Indirect evidence has suggested that circulating trophic factor(s) are involved in intestinal adaptation. A 3T3 fibroblast cell culture system was used to more directly delineate the presence of such factors. Rats were divided into four groups; C-unop, those undergoing no surgery, S, those in which a dorsal slit was made; C-op, those in which the peritoneum was incised; and R, those undergoing 80% intestinal resection. At the time of death at 24 hours, one week and two weeks postoperative, stimulation of DNA synthesis in the 3T3 cells was noted after incubation with platelet free plasma from the C-op and R groups, and simultaneously an increase in ileal DNA specific activity occurred. Characterisation of the plasma fraction with growth factor activity revealed it to have a MW of greater than 6,000 and less than 14,000 d. The factor(s) was resistant to reduction with DTT, and was partially inactivated by heating to 60 degrees C. The use of 3T3 cell growth factor assay system allows further characterisation of circulating factors involved in intestinal adaptation.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Biller et al. (1987) studied Intestinal adaptation following intestinal resection. 80% intestinal resection or peritoneal incision vs. No surgery or dorsal slit was evaluated on Stimulation of DNA synthesis in 3T3 fibroblast cells and ileal DNA specific activity. Platelet-free plasma from rats with 80% intestinal resection or peritoneal incision stimulated 3T3 cell DNA synthesis via 6,000-14,000 Da trophic factors.

synapsesocial.com/papers/6a72483be71d69abee0989b4 https://doi.org/10.1136/gut.28.suppl.243

View Full Paper

Ask AI

Bookmark

Share

Characterisation of humoral trophic factors involved in intestinal adaptation using a 3T3 cell growth factor assay.

Key Points

Structured PICO

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Characterisation of humoral trophic factors involved in intestinal adaptation using a 3T3 cell growth factor assay.

Key Points

Structured PICO

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Keep reading

Keep reading