Key Points
- The aim is to analyze the structure and developmental expression of the chick alpha-actin gene.
- Isolated recombinant DNA clones with chick alpha-actin mRNA sequences.
- Used probes for analyzing expression during chick leg muscle development.
- Identified intervening sequences within the alpha-actin gene from a genomic library.
- Alpha-actin mRNA was present in high abundance in terminally differentiated muscle but low at early stages.
- Detected shorter alpha-actin mRNA forms in fused leg muscle, with sizes less than 2000 nucleotides.
- Found alpha-actin-like mRNA in early embryonic brain, indicating non-muscle-specific transcription.
Structured PICO
PPopulationChick alpha-actin gene and mRNA from in vivo leg muscle and early embryonic brain
IInterventionRecombinant DNA clones containing chick alpha-actin mRNA sequence used as probes
OOutcomeStructure and developmental expression of the chick alpha-actin gene
The chick alpha-actin gene contains multiple short intervening sequences and may not be strictly muscle-specific at all developmental stages.