What question did this study set out to answer?

To determine how cell geometry, internal viscosity, buffer pH, and cell volume influence red blood cell deformability using a standardized filtration passage model.

March 1, 1981Experimental Biology and Medicine

Effect of Cell Geometry, Internal Viscosity, and pH on Erythrocyte Filterability

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Authors

JNJames M. NortonDartmouth College NBNancy D. BarkerBoston Children's Hospital PRPeter W. RandColorado School of Mines

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Implication

In vitro experimental study reveals that surface area-to-volume ratio and internal viscosity dictate filterability in mammalian red blood cells, indicating key physical limits in microcirculation.

Key Points

To determine how cell geometry, internal viscosity, buffer pH, and cell volume influence red blood cell deformability using a standardized filtration passage model.
Measured relative filtration time (RFT) of dilute erythrocyte suspensions passing through 2.8-µm cylindrical pores compared to buffer passage time.
Assessed rat, rabbit, and human erythrocytes across a volume span of 60–100 μm³ while independently manipulating buffer osmolality and pH.
Decreasing the surface area-to-volume ratio via osmotic swelling produced marked increases in RFT, and hypertonic conditions increased RFT nearly linearly with intracellular hemoglobin concentration.
Reducing buffer pH below 7.20 significantly increased RFT through altered membrane properties, whereas baseline volume differences alone accounted for minimal change compared to alterations in surface area-to-volume ratio.

Cite This Study

Norton et al. (1981) studied this question.

synapsesocial.com/papers/6a7287233ce530166bc356ff https://doi.org/10.3181/00379727-166-41089

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Implication

In vitro experimental study reveals that surface area-to-volume ratio and internal viscosity dictate filterability in mammalian red blood cells, indicating key physical limits in microcirculation.

Effect of Cell Geometry, Internal Viscosity, and pH on Erythrocyte Filterability

Discussion

Member takes

Implication

Key Points

Cite This Study

Effect of Cell Geometry, Internal Viscosity, and pH on Erythrocyte Filterability

Discussion

Member takes

Implication

Key Points

Cite This Study

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