August 1, 1968Journal of Cellular Physiology
A study of the intracellular transport of calcium in rat heart
Population
Rat heart model (in vivo and homogenates)
Comparison
In vivo injection of 45 Ca++ with… vs Controls (no PCP)
Design
Preclinical
Authors
Discussion
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Implication
Mitochondrial calcium handling in rodent hearts merits targeted follow-up; leaves open relevance to human myocardium or therapy.
Key Points
- Investigate the intracellular distribution and transport mechanisms of calcium across subcellular fractions in intact rat heart tissue.
- Injected radioactive calcium-45 in vivo into rats and measured its distribution across isolated cardiac subcellular organelles and soluble fractions.
- Administered pentachlorophenol in vivo to uncouple mitochondrial oxidative phosphorylation and assess subsequent shifts in calcium distribution.
- Evaluated non-metabolic binding of calcium-45 in isolated heart homogenates at 0°C with and without pentachlorophenol.
- Mitochondria contained the majority of organelle-associated calcium-45, exhibiting specific activity several times higher than that of the sarcoplasmic reticulum.
- Pentachlorophenol administration significantly depleted mitochondrial calcium-45, causing a corresponding increase in calcium accumulation within microsomes and the soluble supernatant.
- In vitro exposure at 0°C resulted in metabolism-independent calcium binding across subcellular fractions that was unaffected by pentachlorophenol.
Structured PICO
P
Population
Rat heart model (in vivo and homogenates)
I
Intervention
In vivo injection of 45 Ca++ with pentachlorophenol (PCP) to uncouple oxidative phosphorylation
C
Comparator
Controls (no PCP)
O
Outcome
Distribution of 45 Ca++ in subcellular fractions (mitochondria, sarcoplasmic reticulum, microsomes, soluble phase)surrogate
Energy-linked movements of calcium take place in mitochondria of the intact rat heart, which are likely more active than those in the sarcoplasmic reticulum.
Cite This Study
Patriarca et al. (1968) studied this question.