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January 1, 1981Nucleic Acids ResearchOpen Access

Nudeotide sequence at the end of the gene for the RNA polymerase β subunit (rpoC)

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Authors

CSCatherine L. SquiresTufts University AKAdrian R. KrainerCold Spring Harbor Laboratory GBGerard F. BarryMonsanto (United States)

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Implication

Analysis uncovers transcription termination and open reading frames in E. coli, suggesting functional implications.

Key Points

This research aims to identify the DNA sequence surrounding the transcription terminator of the rpoC gene in E. coli.
Sequenced the distal sections of the rpoC gene and surrounding regions (2044 bp)
Utilized S1 mapping to locate the 3' end of rpoC mRNA
Identified secondary structures and open reading frames in the sequence
Located the major transcription termination occurring shortly past the rpoC stop codon
Identified four symmetrical regions, indicating potential secondary structures in the mRNA
Noted that ORFa and ORFb contained codons recognized by minor tRNA species, while rpoC favored major tRNA

Cite This Study

Squires et al. (1981) studied this question.

synapsesocial.com/papers/6a72b6c8f44fa9f079e017dc https://doi.org/10.1093/nar/9.24.6827

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