December 3, 2020Arteriosclerosis Thrombosis and Vascular BiologyOpen Access

Nonredundant Roles of Platelet Glycoprotein VI and Integrin αIIbβ3 in Fibrin-Mediated Microthrombus Formation

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Why the study?

Fibrin is considered to strengthen thrombus formation via integrin αIIbβ3, but recent findings indicate it can also act as a ligand for platelet glycoprotein VI.

Population

Whole blood flowed over immobilized fibrin surfaces, including blood from Glanzmann thrombasthenia

Comparison

Inhibition or absence of αIIbβ3, glycoprotein VI, or Syk vs uninhibited conditions

Design

In vitro microfluidics and suspension assay study

Authors

GPGina PerrellaUniversity College Birmingham JHJingnan HuangUniversity of Electronic Science and Technology of China IPIsabella ProvenzaleMaastricht University Medical Centre

Discussion

Member takes

Implication

Fibrin may consolidate microthrombi via GPVI–αIIbβ3 interplay; leaves open whether dual inhibition alters clinical thrombosis risk.

Structured PICO

Population

Whole blood flowed at high shear rate (1000 s-1) over immobilized fibrin surfaces, including blood with Glanzmann thrombasthenia

Intervention

Blocking of glycoprotein VI (with Fab 9O12), inhibition of Syk, or absence of αIIbβ3

Comparator

Unblocked/normal conditions

Outcome

Platelet adhesion, activation (P-selectin expression), secretion, Ca2+ signaling, aggregation, and microthrombus formationsurrogate

Fibrin mediates microthrombus formation through nonredundant roles of platelet glycoprotein VI and integrin αIIbβ3, highlighting potential mechanistic targets for antithrombotic therapy.

Cite This Study

Perrella et al. (2020) studied this question.

synapsesocial.com/papers/6a72c0b7e5469ee92be28c8c https://doi.org/10.1161/atvbaha.120.314641

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December 3, 2020Arteriosclerosis Thrombosis and Vascular BiologyOpen Access

Nonredundant Roles of Platelet Glycoprotein VI and Integrin αIIbβ3 in Fibrin-Mediated Microthrombus Formation

View Full Paper

Ask AI

Bookmark

Why the study?

Fibrin is considered to strengthen thrombus formation via integrin αIIbβ3, but recent findings indicate it can also act as a ligand for platelet glycoprotein VI.

Population

Whole blood flowed over immobilized fibrin surfaces, including blood from Glanzmann thrombasthenia

Comparison

Inhibition or absence of αIIbβ3, glycoprotein VI, or Syk vs uninhibited conditions

Design

In vitro microfluidics and suspension assay study

Authors

GPGina PerrellaUniversity College Birmingham JHJingnan HuangUniversity of Electronic Science and Technology of China IPIsabella ProvenzaleMaastricht University Medical Centre

Discussion

Member takes

Implication

Fibrin may consolidate microthrombi via GPVI–αIIbβ3 interplay; leaves open whether dual inhibition alters clinical thrombosis risk.

Structured PICO

Population

Whole blood flowed at high shear rate (1000 s-1) over immobilized fibrin surfaces, including blood with Glanzmann thrombasthenia

Intervention

Blocking of glycoprotein VI (with Fab 9O12), inhibition of Syk, or absence of αIIbβ3

Comparator

Unblocked/normal conditions

Outcome

Platelet adhesion, activation (P-selectin expression), secretion, Ca2+ signaling, aggregation, and microthrombus formationsurrogate

Fibrin mediates microthrombus formation through nonredundant roles of platelet glycoprotein VI and integrin αIIbβ3, highlighting potential mechanistic targets for antithrombotic therapy.

Cite This Study

Perrella et al. (2020) studied this question.

synapsesocial.com/papers/6a72c0b7e5469ee92be28c8c https://doi.org/10.1161/atvbaha.120.314641

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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