Method for the Determination or Rates of Spore Inactivation at Ultra-High Temperatures

In a procedure for determining the rates of destruction of heat-resistant bacterial spores at temperatures between 119-130 C in 3:1 whole milk concentrate or 0.067 ~ phosphate buffer, thermal inactivation tubes (Figure ]~aeh tube was sealed and constricted in an oxygen flame about 15 mm from one end, to facilitate opening the tube and removing its contents at the time of subeulturing. Sterilized tubes were filled aseptically by means