Why the study?
Existing protocols for isolating myofibrillar proteins from skeletal muscle often face technical challenges achieving complete resuspension of the myofibril pellet.
Population
Rodent and human skeletal muscle
Comparison
Several previously published MF isolation methods
Authors
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Optimized MF isolation eases pellet resuspension; leaves open cardiac muscle validation before broader use.
Provides an optimized protocol for isolating and solubilizing myofibrillar proteins from skeletal muscle, overcoming technical challenges of previous methods.
Roberts et al. (2020) studied this question.
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