Randomized trial investigates DMH neuron roles in post-anesthesia sleep disturbance, indicating new therapeutic targets.
Key Points
This study explores the distinct roles of DMH glutamatergic and GABAergic neurons in regulating post-anesthesia sleep disturbance and their mechanisms.
Mouse model of post-anesthesia sleep disturbance induced by isoflurane anesthesia was used.
Chemogenetics to activate or inhibit DMH neurons; sleep–wake architecture was assessed using EEG and EMG biotelemetry.
Single-nucleus RNA sequencing provided molecular profiling of neuronal activity.
Isoflurane-induced sleep disturbance led to increased wakefulness and fragmented sleep during recovery.
Activation of DMH glutamatergic neurons reversed post-anesthesia sleep disturbance, while modulation of DMH GABA neurons also improved PSD.
CB1R antagonist administration alleviated PSD, indicating a role for cannabinoid signaling in sleep regulation.