This preclinical study demonstrates the feasibility of collecting heart lymph in a dog model to study capillary permeability and macromolecule transport.
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Feasibility of steady cardiac lymph collection shown in dogs; leaves open translation to human capillary permeability studies.
For some time we have been interested in problems of surgical shock and the maintenance of an adequate circulating blood volume. The ultimate aim was to find out some of the characteristics of the 'ideal' plasma-expander molecule. This led us to study the permeability of the capillary membrane, the pro-duction of lymph, the return of the proteins from the extravascular space to the circulating blood and related problems. In the earlier studies lymph from the leg, the liver and the cervical region was collected, and the transport of various molecular sizes of dextran from plasma to lymph from these regions was measured (Grotte, 1956; Arturson, 1961). During these studies we found that lymph from the heart could be successfully collected. Lymph flows in considerable quantities from this organ and the flow is quite steady for several hours. In some experiments we also used the classical Starling heart-lung preparation (Areskog, 1962) on a dog in which we had cannulated one of the lymphatic trunks coming from the heart. As test substance, we used dextran fractions of suitable molecular distributions, with molecular weights ranging from 5,200-350,000 and also serum albumin tagged with 1311 or 1251.
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Areskog et al. (1964) studied this question.
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