March 1, 1998AJP Cell Physiology

Cell-specific promoter in adenovirus vector for transgenic expression of SERCA1 ATPase in cardiac myocytes

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Population

Cultured chick embryo cardiac myocytes and fibroblasts

Comparison

Adenovirus-mediated transfer of cDNA encoding… vs Control myocytes

Design

Preclinical

Authors

GIGiuseppe InesiElectrophysiology DLDavid E. LewisUniversity of Wisconsin–Eau Claire CSCarlota SumbillaUniversity of Maryland, Baltimore

Discussion

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Implication

Demonstrates functional SERCA1 overexpression in myocytes; hypothesis-generating for cardiac gene therapy, pending in vivo validation.

Structured PICO

Population

Cultured chick embryo cardiac myocytes and fibroblasts

Intervention

Adenovirus-mediated transfer of cDNA encoding chicken skeletal muscle sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA1) under control of a segment (-268 base pair) of the cell-specific cardiac troponin T (cTnT) promoter or a constitutive viral (CMV) promoter

Comparator

Control myocytes

Outcome

Transgenic expression of SERCA1 ATPase and ATP-dependent Ca2+ transport activitysurrogate

A cell-specific cTnT promoter in recombinant adenovirus vectors yields highly efficient and selective transgene expression of functional SERCA1 enzyme in cardiac myocytes.

Cite This Study

Inesi et al. (1998) studied this question.

synapsesocial.com/papers/6a73126c542cb04b60cd5b65 https://doi.org/10.1152/ajpcell.1998.274.3.c645

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Cell-specific promoter in adenovirus vector for transgenic expression of SERCA1 ATPase in cardiac myocytes

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Cell-specific promoter in adenovirus vector for transgenic expression of SERCA1 ATPase in cardiac myocytes

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider