Antibacterial effects of 4 methanol plant extracts (Lamiaceae) against 10 Methicillin Resistant Staphylococcus aureus isolates

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Antibiotic resistance has paved the way for replacing conventional medications with herbal therapies that supposedly have less side-effect. This research is an experimental study and a fundamental- functional one. The present study aimed to investigate the antibacterial effect of 4 plant extracts from Lamiaceae on 10 methicillin resistant Staphylococcus aureus isolates. Methanol extracts were prepared by maceration method for 10 days in room temperature and then filtered with wathman paper No.1 and concentrated by rotary evaporator system. Different concentration of each extract were prepared in dimethyl sulfoxide: methanol (1:1 v/v) and bioassayed on 10 MRSA isolates by agar well diffusion method in Muller-Hinton agar medium. Plates were incubated in 37°C for 24 hours. After incubation period, zone of inhibition was measured in millimeter and the antibacterial effect of extracts were evaluated. According to antibiogram test, some of Staphylococcus aureus isolates were sensitive to the used extracts with different MIC.MIC values about all Staphylococcus aureus isolates about Zataria multiflora, Mentha longifolia, Ziziphor clinopodioide and Satureja hortensis were 15, 30, 30 and 15 mg/ml respectively. Because of regarding the fact that antibiotic resistance is growing, according to the acquired results we expect to be able to use plant extracts against Staphylococcus aureus resistant to meticillin in controling the infections or as preservatives in food sciences and in the next step separating of effective substances were suggested.DOI: http://dx.doi.org/10.3126/ijls.v9i2.12045 International Journal of Life Sciences 9 (2) : 2015; 51-54