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March 15, 1992Proceedings of the National Academy of SciencesOpen Access

Detection of intrahepatic replication of hepatitis C virus RNA by in situ hybridization and comparison with histopathology.

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Population

Chimpanzees experimentally infected with the H strain of hepatitis C virus, along with control specimens…

Comparison

Nonisotopic in situ hybridization assay using a… vs Pre-inoculation liver biopsy specimens…

Design

Preclinical

Authors

FNFrancesco NegroUniversity of BresciaDPDonatella PacchioniAzienda Ospedaliera Citta' della Salute e della Scienza di TorinoYSYohko K. ShimizuKobe University

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Implication

Early HCV detection without necrosis cautions against relying solely on serum markers; leaves open translation to human acute infection monitoring.

Structured PICO

P
Population
Chimpanzees experimentally infected with the H strain of hepatitis C virus (HCV) (n=2), along with control specimens from chimpanzees with acute hepatitis A, B, or delta, and uninfected animals.
I
Intervention
Nonisotopic in situ hybridization (NISH) assay using a synthetic oligonucleotide complementary to bases 252-301 of the 5' noncoding region of the HCV genome.
C
Comparator
Pre-inoculation liver biopsy specimens, specimens from chimpanzees with other hepatitis types, uninfected animals, RNase predigested specimens, and hybridization with an unrelated labeled oligomer.
O
Outcome
Intracellular distribution of HCV RNA in the liver compared with serum alanine aminotransferase activity, serum HCV RNA, and liver histopathology.surrogate

Nonisotopic in situ hybridization is a sensitive and specific method for detecting intracellular HCV replication, which correlates with early liver enzyme elevation prior to histopathologic changes.

Cite This Study

Negro et al. (1992) studied this question.

synapsesocial.com/papers/6a7324f5ef2d17db5ca0baa2https://doi.org/10.1073/pnas.89.6.2247
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Also Consider

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  1. 1Effect of Interferon on Mouse Cells Chronically Infected with Murine Leukaemia Virus: Kinetic Studies on Virus Production and Virus RNA Synthesis1978 · 23 citations
  2. 2Nucleotide sequence and mutation rate of the H strain of hepatitis C virus.1991 · 610 citations
  3. 3Characterization of the terminal regions of hepatitis C viral RNA: identification of conserved sequences in the 5' untranslated region and poly(A) tails at the 3' end.1991 · 427 citations
  4. 4Isolation of a cDNA cLone Derived from a Blood-Borne Non-A, Non-B Viral Hepatitis Genome1989 · 6,913 citations
  5. 5Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes).1984 · 3,208 citations