What question did this study set out to answer?

To determine whether membrane progesterone receptors are expressed in human peripheral blood leukocytes and characterize their signaling functions in T lymphocytes and Jurkat cells.

October 5, 2007Journal of Endocrinology

Expression of membrane progesterone receptors on human T lymphocytes and Jurkat cells and activation of G-proteins by progesterone

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Authors

CDChrysoula DosiouStanford University AHAmy E. HamiltonMaine Department of Marine Resources YPYefei PangThe University of Texas at Austin

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Implication

Laboratory study demonstrates membrane progesterone receptors on human T lymphocytes, suggesting a direct mechanism for hormone-mediated immune regulation.

Key Points

To determine whether membrane progesterone receptors are expressed in human peripheral blood leukocytes and characterize their signaling functions in T lymphocytes and Jurkat cells.
Measured mPRalpha, mPRbeta, and mPRgamma transcript and protein expression in peripheral blood leukocytes, T lymphocytes, and Jurkat cells using RT-PCR and Western blotting.
Assessed steroid membrane receptor binding with [(3)H]progesterone radioreceptor assays and tested progesterone-stimulated inhibitory G-protein (Gi) activation.
Transcripts and membrane proteins for mPRalpha and mPRbeta, but not mPRgamma, were identified in peripheral blood leukocytes, T lymphocytes, and Jurkat cells.
Expression of mPRalpha mRNA was selectively upregulated during the luteal phase of the menstrual cycle in CD8+ T lymphocytes, with no change observed in CD4+ cells.
Progesterone exhibited specific membrane binding with a dissociation constant (Kd) of 4.25 nM and activated an inhibitory G-protein (Gi) in Jurkat cells.

Cite This Study

Dosiou et al. (2007) studied this question.

synapsesocial.com/papers/6a739bd02f7fe9cfa171b9bc https://doi.org/10.1677/joe-07-0317

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Implication

Laboratory study demonstrates membrane progesterone receptors on human T lymphocytes, suggesting a direct mechanism for hormone-mediated immune regulation.

Expression of membrane progesterone receptors on human T lymphocytes and Jurkat cells and activation of G-proteins by progesterone

Discussion

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Implication

Key Points

Cite This Study

Expression of membrane progesterone receptors on human T lymphocytes and Jurkat cells and activation of G-proteins by progesterone

Discussion

Member takes

Implication

Key Points

Cite This Study

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