What question did this study set out to answer?

To resolve manganese-induced interference and false elevations in enzymatic cholesterol measurements of high-density lipoprotein (HDL) fractions.

January 1, 1976Clinical Chemistry

Enzymatic determinations of cholesterol in high-density-lipoprotein fractions prepared by a precipitation technique.

View Full Paper

Ask AI

Bookmark

Authors

BSBernard W. SteeleUniversity of Miami DKDonald F. KoehlerUniversity of Münster MAM M AzarUniversity of Minnesota

Discussion

Member takes

Implication

Laboratory evaluation demonstrates an EDTA-modified enzymatic assay accurately measures HDL cholesterol in manganese-precipitated serum, suggesting a reliable single-run protocol for lipid profiling.

Key Points

To resolve manganese-induced interference and false elevations in enzymatic cholesterol measurements of high-density lipoprotein (HDL) fractions.
Reconstituted an enzymatic cholesterol reagent with an ethylenediaminetetraacetate (EDTA) solution to eliminate turbidity caused by manganese precipitation in serum HDL fractions.
Validated analytical accuracy against the Lipid Research Clinic reference procedure using identical lipoprotein preparations.
Assessed intra-day and overall precision while evaluating reagent linearity for total cholesterol measurements exceeding 4 g/L.
The modified assay showed strong agreement with the Lipid Research Clinic procedure (regression slope = 0.998, Y-intercept = 8.9 mg/L, correlation coefficient r = 0.984, standard error of the estimate = 16.8 mg/L).
Precision metrics including the precipitation step demonstrated a within-day standard deviation of 9.7 mg/L and an overall standard deviation of 23.7 mg/L.
Linearity extended beyond 4 g/L total cholesterol, allowing concurrent analysis of HDL and total cholesterol within a single analytical run.

Cite This Study

Steele et al. (1976) studied this question.

synapsesocial.com/papers/6a73b19f9d09e2fbac27a0df https://doi.org/10.1093/clinchem/22.1.98

View Full Paper

Ask AI

Bookmark

January 1, 1976Clinical Chemistry

Enzymatic determinations of cholesterol in high-density-lipoprotein fractions prepared by a precipitation technique.

View Full Paper

Ask AI

Bookmark

Authors

BSBernard W. SteeleUniversity of Miami DKDonald F. KoehlerUniversity of Münster MAM M AzarUniversity of Minnesota

Discussion

Member takes

Implication

Laboratory evaluation demonstrates an EDTA-modified enzymatic assay accurately measures HDL cholesterol in manganese-precipitated serum, suggesting a reliable single-run protocol for lipid profiling.

Key Points

To resolve manganese-induced interference and false elevations in enzymatic cholesterol measurements of high-density lipoprotein (HDL) fractions.
Reconstituted an enzymatic cholesterol reagent with an ethylenediaminetetraacetate (EDTA) solution to eliminate turbidity caused by manganese precipitation in serum HDL fractions.
Validated analytical accuracy against the Lipid Research Clinic reference procedure using identical lipoprotein preparations.
Assessed intra-day and overall precision while evaluating reagent linearity for total cholesterol measurements exceeding 4 g/L.
The modified assay showed strong agreement with the Lipid Research Clinic procedure (regression slope = 0.998, Y-intercept = 8.9 mg/L, correlation coefficient r = 0.984, standard error of the estimate = 16.8 mg/L).
Precision metrics including the precipitation step demonstrated a within-day standard deviation of 9.7 mg/L and an overall standard deviation of 23.7 mg/L.
Linearity extended beyond 4 g/L total cholesterol, allowing concurrent analysis of HDL and total cholesterol within a single analytical run.

Cite This Study

Steele et al. (1976) studied this question.

synapsesocial.com/papers/6a73b19f9d09e2fbac27a0df https://doi.org/10.1093/clinchem/22.1.98

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark