A line-shaped human cardiomyocyte network improves the uniformity and stability of electrophysiological parameters compared to conventional clusters, offering a more reproducible model for predictive drug-induced cardiotoxicity.
May improve reproducibility of in vitro cardiotoxicity models; hypothesis-generating and should not yet alter screening practices.
We have evaluated the electrophysiological characteristics of a line-shaped network of a three-dimensionally controlled in vitro human cardiomyocyte assay (hCM line) against conventional cell clusters as the standard model (hCM cluster) from the viewpoint of quality control of sample variety and time–course stability. The beating intervals of the hCM line demonstrated a more stable uniformity of samples (846 ±130 ms, 15.3% fluctuation) and better time–course stability, whereas those of the hCM cluster showed a much larger variety of samples (2001 ±1127 ms, 56.3% fluctuation) and weaker time–course stability. The field potential amplitude of the hCM line also showed better uniformity of samples (629 ±428 µV, 68.0% fluctuation) against those of the hCM cluster (1984 ±2288 µV, 115.3% fluctuation). The results suggested the importance of the cell-network shape control for the uniformity and stability of the beating interval and the field potential amplitude. They also suggest that the hCM line can improve the reproducibility and accuracy of the samples, which is important for a functional human cardiotoxicity model.
No takes yet. Share an insight, caveat, or question.
Hamada et al. (2013) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: