August 1, 1990The Journal of Cell BiologyOpen Access

Subtilisin cleavage of actin inhibits in vitro sliding movement of actin filaments over myosin.

View Full Paper

Ask AI

Bookmark

Population

In vitro actin filaments and heavy meromyosin (HMM)

Comparison

Subtilisin cleavage of actin vs Intact actin

Design

Preclinical

Authors

DSD H SchwyterUniversity of California, Los Angeles SKStephen J. KronUniversity of Chicago YTYoko Y. ToyoshimaThe University of Tokyo

Discussion

Member takes

Implication

Actin structural integrity is required for filament motility; extends mechanistic insights into actomyosin function but leaves relevance to cardiac contractility open.

Structured PICO

Population

In vitro actin filaments and heavy meromyosin (HMM)

Intervention

Subtilisin cleavage of actin

Comparator

Intact actin

Outcome

In vitro sliding movement (motility) over an HMM coated surface

Subtilisin cleavage of actin uncouples the motility of actin filaments from the activation of myosin ATPase activity, indicating that motility depends on actin's structural integrity.

Cite This Study

Schwyter et al. (1990) studied this question.

synapsesocial.com/papers/6a73bf3914e943abc869f122 https://doi.org/10.1083/jcb.111.2.465

View Full Paper

Ask AI

Bookmark

Implication

Actin structural integrity is required for filament motility; extends mechanistic insights into actomyosin function but leaves relevance to cardiac contractility open.

Subtilisin cleavage of actin inhibits in vitro sliding movement of actin filaments over myosin.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Subtilisin cleavage of actin inhibits in vitro sliding movement of actin filaments over myosin.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider