Phytochemicals and GC-MS analysis of fatty acids in leaves of Acmella caulirhiza traditional medicinal plant, evaluating efficiencies of extracting solvents and methods
Experimental study demonstrates superior phytochemical and fatty acid extraction using methanol Soxhlet methods in Acmella caulirhiza leaves, highlighting its therapeutic potential.
Key Points
To screen phytochemical constituents in the leaves of Acmella caulirhiza and determine the extraction efficiencies of four distinct organic solvents across two extraction methods.
Evaluated maceration and Soxhlet extraction techniques using methanol, ethanol, acetone, and petroleum ether solvents.
Quantified total phenol and flavonoid concentrations and evaluated differences in extraction efficiency across solvent polarities using ANOVA.
Identified and profiled fatty acid constituents using gas chromatography-mass spectrometry (GC-MS).
Methanol Soxhlet extraction produced the maximum extraction yield of 13.2% (compared to 8.2% via maceration), yielding a total phenol content of 22.10 ± 2.05 mg g−1 and total flavonoid content of 9.71 ± 1.45 mg g−1.
GC-MS analysis revealed more than 10 fatty acids, dominated by hexadecenoic acid at 38.5% and linoleic acid at 34.21% of total fatty acid content.