What question did this study set out to answer?

To determine the association of PKC isoforms with cardiac troponin and investigate how PKCzeta regulates myofilament protein phosphorylation.

August 28, 2007Journal of Biological ChemistryOpen Access

Protein Kinase C ζ

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Population

Adult cardiac myocytes

Comparison

Adenoviral constructs containing DsRed… vs Wild type (WT) PKCzeta

Design

Preclinical

Authors

SWSteven C. WuUniversity of Minnesota RSR. John SolaroGeneral Cardiology

Discussion

Member takes

Implication

PKCzeta emerges as a myofilament regulator in rodent myocytes; leaves open its role in human cardiac contractility or therapy.

Key Points

To determine the association of PKC isoforms with cardiac troponin and investigate how PKCzeta regulates myofilament protein phosphorylation.
Transduced adult cardiac myocytes with adenoviral vectors expressing wild-type or constitutively active PKCzeta mutants (A119E, T410E, and T560E).
Evaluated subcellular localization to Z-discs and quantified phosphorylation of cTnI, cTnT, tropomyosin, myosin-binding protein C, and desmin via immunoprecipitation.
Tested for macromolecular complex formation between PKCzeta, p21-activated kinase-1 (Pak1), and protein phosphatase 2A (PP2A) using co-immunoprecipitation Western blotting.
Active PKCzeta mutants (notably T560E) decreased threonine phosphorylation of cTnI and cTnT, whereas all three mutants increased serine phosphorylation of tropomyosin.
PKCzeta A119E increased myosin-binding protein C phosphorylation, and both A119E and T410E localized to Z-discs and increased desmin phosphorylation.
PKCzeta co-immunoprecipitated with Pak1 and PP2A, demonstrating a multi-protein complex that accounts for the dephosphorylation of cTnI and cTnT.

Structured PICO

Population

Adult cardiac myocytes

Intervention

Adenoviral constructs containing DsRed monomer-tagged wild type (WT) and constitutively active forms of PKCzeta (A119E, T410E, T560E)

Comparator

Wild type (WT) PKCzeta

Outcome

Phosphorylation of cTnI, cTnT, tropomyosin, myosin-binding protein C, and desminsurrogate

PKCzeta is a novel regulator of myofilament protein phosphorylation in cardiac myocytes, likely acting through a complex with Pak1 and PP2A.

Cite This Study

Wu et al. (2007) studied this question.

synapsesocial.com/papers/6a73d6d243194bd21bf15cae https://doi.org/10.1074/jbc.m703670200

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Protein Kinase C ζ

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Protein Kinase C ζ

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider