Activation of Myosin Light Chain Kinase Requires Translocation of Bound Calmodulin

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Population

Skeletal muscle myosin light chain kinase and calmodulin (CaM) proteins

Comparison

Mutant of CaM missing residues 2-8 (DeltaNCaM) vs Native calmodulin (CaM)

Design

Preclinical

Authors

Discussion

Animal findings on DeltaNCaM do not inform cardiac myosin kinase regulation; leaves open whether N-terminal residues are essential in myocardium.

Population

Skeletal muscle myosin light chain kinase and calmodulin (CaM) proteins

Intervention

Mutant of CaM missing residues 2-8 (DeltaNCaM)

Comparator

Native calmodulin (CaM)

Outcome

Activation of catalysis and structural position (translocation) measured by small angle x-ray scatteringsurrogate

Calmodulin residues 2-8 are essential for the translocation step required to activate skeletal muscle myosin light chain kinase.

Krueger et al. (2001) studied this question.

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Animal findings on DeltaNCaM do not inform cardiac myosin kinase regulation; leaves open whether N-terminal residues are essential in myocardium.