What question did this study set out to answer?

To determine the in vivo developmental role of the type III transforming growth factor beta receptor (TbetaRIII) and assess how its absence affects ligand responsiveness.

May 28, 2003Molecular and Cellular BiologyOpen Access

Heart and Liver Defects and Reduced Transforming Growth Factor β2 Sensitivity in Transforming Growth Factor β Type III Receptor-Deficient Embryos

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Authors

KSKaye L. StenversHudson Institute of Medical Research MTMelinda L. TurskyUNSW Sydney KHKenneth W. HarderUniversity of British Columbia

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Implication

Murine knockout study reveals lethal heart and liver defects alongside reduced TGF-beta2 sensitivity in TbetaRIII-null embryos, indicating an essential role in midgestation organ development.

Key Points

To determine the in vivo developmental role of the type III transforming growth factor beta receptor (TbetaRIII) and assess how its absence affects ligand responsiveness.
Identified midgestation murine expression patterns and disrupted the TbetaRIII gene via homologous recombination.
Assessed embryonic phenotypes starting at embryonic day 13.5 and generated primary embryonic fibroblasts to evaluate ligand sensitivity, growth inhibition, reporter gene activation, and Smad2 nuclear localization.
TbetaRIII-null mouse embryos developed lethal proliferative cardiac defects and liver apoptosis beginning at embryonic day 13.5.
TbetaRIII-deficient primary fibroblasts showed selective, significant reductions in sensitivity to TGF-beta2 for growth inhibition, reporter gene activation, and Smad2 nuclear localization compared to wild-type cells, with responses to other TGF-beta ligands preserved.

Cite This Study

Stenvers et al. (2003) studied this question.

synapsesocial.com/papers/6a73e10176f4fb0e4269f3c2 https://doi.org/10.1128/mcb.23.12.4371-4385.2003

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Implication

Murine knockout study reveals lethal heart and liver defects alongside reduced TGF-beta2 sensitivity in TbetaRIII-null embryos, indicating an essential role in midgestation organ development.

Heart and Liver Defects and Reduced Transforming Growth Factor β2 Sensitivity in Transforming Growth Factor β Type III Receptor-Deficient Embryos

Discussion

Member takes

Implication

Key Points

Cite This Study

Heart and Liver Defects and Reduced Transforming Growth Factor β2 Sensitivity in Transforming Growth Factor β Type III Receptor-Deficient Embryos

Discussion

Member takes

Implication

Key Points

Cite This Study

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