What question did this study set out to answer?

To determine whether leukotriene D4 stimulates prostanoid synthesis by modulating phospholipase A2 or phospholipase C activity in vascular cells.

August 1, 1986Journal of Biological ChemistryOpen Access

Leukotriene D4 treatment of bovine aortic endothelial cells and murine smooth muscle cells in culture results in an increase in phospholipase A2 activity.

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Authors

MCM A ClarkUnited States Department of Veterans Affairs DLDavid LittlejohnUniversity of Strathclyde TCTheresa ConwayAgency for Science, Technology and Research

Discussion

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Implication

Cell culture study demonstrates that leukotriene D4 stimulates phospholipase A2 activity in vascular cells, indicating that phospholipase A2 drives downstream prostanoid synthesis.

Key Points

To determine whether leukotriene D4 stimulates prostanoid synthesis by modulating phospholipase A2 or phospholipase C activity in vascular cells.
Cultured bovine aortic endothelial cells and murine smooth muscle cells were exposed to leukotriene D4 treatment.
Assayed phospholipase A2 activity using phosphatidylcholine as a substrate, tested phospholipase C activity across substrates, and evaluated the dependence on RNA and protein synthesis.
Leukotriene D4 treatment produced a dose-dependent, stereospecific increase in phospholipase A2 activity driven by an elevated apparent Vmax prior to prostanoid release.
The increase in phospholipase A2 activity required de novo RNA and protein synthesis, whereas phospholipase C activity remained unaltered.

Cite This Study

Clark et al. (1986) studied this question.

synapsesocial.com/papers/6a7402360e7fa2a8810dde60 https://doi.org/10.1016/s0021-9258(18)67444-7

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Implication

Cell culture study demonstrates that leukotriene D4 stimulates phospholipase A2 activity in vascular cells, indicating that phospholipase A2 drives downstream prostanoid synthesis.

Leukotriene D4 treatment of bovine aortic endothelial cells and murine smooth muscle cells in culture results in an increase in phospholipase A2 activity.

Discussion

Member takes

Implication

Key Points

Cite This Study

Leukotriene D4 treatment of bovine aortic endothelial cells and murine smooth muscle cells in culture results in an increase in phospholipase A2 activity.

Discussion

Member takes

Implication

Key Points

Cite This Study

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