What question did this study set out to answer?

Investigate how membrane depolarization and extracellular acidification alter the recovery kinetics of sodium channels from inactivation and lidocaine block in cardiac cells.

December 1, 1988AJP Heart and Circulatory Physiology

pH and voltage dependence of INa recovery kinetics in atrial cells exposed to lidocaine

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Why the study?

How do pH and voltage affect the recovery kinetics of sodium channels in atrial cells exposed to lidocaine?

Population

Atrial cells isolated from the bullfrog

Comparison

Lidocaine exposure under varying pH and voltage… vs Drug-free conditions

Design

Preclinical

Authors

KCKenneth R. CourtneyAcademy of Medical Sciences

Discussion

Member takes

Implication

Does not support altering lidocaine use; leaves open clinical relevance of pH-voltage effects on sodium channel recovery.

Key Points

Investigate how membrane depolarization and extracellular acidification alter the recovery kinetics of sodium channels from inactivation and lidocaine block in cardiac cells.
Conducted single-electrode voltage-clamp recordings on isolated atrial cells obtained from bullfrogs.
Measured sodium current repriming kinetics across varying membrane potentials and pH conditions with and without lidocaine exposure.
Membrane depolarization and extracellular acidification each slowed the rate of sodium channel recovery from lidocaine block.
Sodium channel recovery from inactivation under drug-free conditions accelerated at higher pH levels, displaying the opposite pH response observed in myelinated nerves.
Recovery kinetics in the presence of lidocaine indicated that channels bound to neutral drug molecules reactivate fastest, coupled to recovery from channel inactivation.

Structured PICO

How do pH and voltage affect the recovery kinetics of sodium channels in atrial cells exposed to lidocaine?

Population

Atrial cells isolated from the bullfrog

Intervention

Lidocaine exposure under varying pH and voltage conditions

Comparator

Drug-free conditions

Outcome

Modulation of recovery kinetics (repriming) of drug-blocked sodium channelssurrogate

This preclinical study elucidates the biophysical mechanisms by which pH and voltage modulate the recovery of cardiac sodium channels from lidocaine block.

Cite This Study

Kenneth R. Courtney (1988) studied this question.

synapsesocial.com/papers/6a742457e50bdaa0a433f854 https://doi.org/10.1152/ajpheart.1988.255.6.h1554

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pH and voltage dependence of INa recovery kinetics in atrial cells exposed to lidocaine

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

pH and voltage dependence of INa recovery kinetics in atrial cells exposed to lidocaine

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider