February 1, 1988Journal of VirologyOpen Access

Direct measurement of the poliovirus RNA polymerase error frequency in vitro

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Population

In vitro model using purified poliovirus RNA-dependent RNA polymerase and homopolymeric RNA templates (poly…

Comparison

Copying RNA templates with equimolar… vs Different reaction conditions, templates, or…

Design

Preclinical

Authors

CWCarol D. WardNorthwestern University MSMary A. M. StokesUniversity of Florida JFJames B. FlaneganUniversity of Florida

Discussion

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Implication

High poliovirus polymerase error rates may accelerate viral evolution; leaves open in vivo effects of Mg2+ and pH on fidelity.

Structured PICO

Population

In vitro model using purified poliovirus RNA-dependent RNA polymerase and homopolymeric RNA templates (poly(A), poly(C), poly(I))

Intervention

Copying RNA templates with equimolar concentrations of noncomplementary and complementary ribonucleotides under varying reaction conditions (e.g., 3.0 mM Mg2+ pH 7.0 vs 7.0 mM Mg2+ pH 8.0)

Comparator

Different reaction conditions, templates, or substrates

Outcome

Error frequency (amount of noncomplementary nucleotide incorporated divided by total complementary and noncomplementary nucleotide incorporated)surrogate

The poliovirus RNA-dependent RNA polymerase exhibits a high error frequency in vitro, which increases under conditions that promote a faster elongation rate.

Cite This Study

Ward et al. (1988) studied this question.

synapsesocial.com/papers/6a746cddf5fc39414403235b https://doi.org/10.1128/jvi.62.2.558-562.1988

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Direct measurement of the poliovirus RNA polymerase error frequency in vitro

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Direct measurement of the poliovirus RNA polymerase error frequency in vitro

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider