Spores of Clostridium botulinum type E have been screened for resistance to heating in aqueous suspension at 60, 70 and 80°. D 176 ranged from <0·33 min to 1·25 min. z values of eight strains were between 9·0 and 10·7, and for one strain was 7·4. Radiation survivor curves have been plotted for nine strains, and a further nine strains screened. The “shoulder” extends to about 0·4 megarad, whereafter the curve becomes exponential within a D value from 0·065 to 0·16 megarad.
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Roberts et al. (1965) studied this question.
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